Influence of the anesthetic technique on circulating extracellular vesicles in bladder cancer patients undergoing radical cystectomy

  • Anesthetics have been shown to alter tumor progression and seem to influence surgical cancer outcome. Circulating extracellular vesicles as mediators of intercellular communication are involved in cancer progression and may be influenced by anesthetics. In this prospective, randomized study, effects of anesthetics on extracellular vesicles and associated micro-RNAs in bladder cancer patients undergoing radical cystectomy were tested. Extracellular vesicles from 51 patients at four perioperative time points receiving Propofol or Sevoflurane were extracted with polymer-based methods and quantified with a nanoparticle-tracking analysis. Vesicle-associated micro-RNAs were analyzed with a real-time polymerase chain reaction using array cards and single assays for tumor-associated miR-21-5p, miR-15a-5p, miR-17-5p and miR-451a. Plasma extracellular vesicle concentration (suture: fold change (fc) in Propofol at 4.1 \(\pm\) 3.9 vs. Sevoflurane at 0.8 \(\pm\) 0.5; p = 0.003) and associated miRNAs increased significantly (+30% post induction, +9% 30 Min surgery) in the Propofol group. Tumor-associated miRNAs increased during surgery in both groups (fc in miR-21-5p: 24.3 \(\pm\) 10.2, \(\it p\) = 0.029; fc in miR-15a-5p: 9.7 \(\pm\) 3.8, \(\it p\) = 0.027; fc in miR-17-5p: 5.4 \(\pm\) 1.7, \(\it p\) = 0.014), whereas antitumor miR-451a increased in the Propofol group only (fc: 2.5 \(\pm\) 0.6 vs. 1.0 \(\pm\) 0.2; \(\it p\) = 0.022). Anesthetics influence extracellular vesicles and associated micro-RNAs of bladder cancer patients during surgery. Increased expression of antitumor micro-RNA may be an explanatory approach for decreased tumor cell viability after Propofol.

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Author:Luisa GluthORCiDGND, Crista OchsenfarthORCiDGND, Phuong Nam Viet PhamGND, Jan M. WischermannGND, Thomas KomanekGND, Florian Bernhard Gerhard RoghmannGND, Ulrich FreyORCiDGND
Parent Title (English):Cells
Subtitle (English):a prospective, randomized trial
Place of publication:Lausanne
Document Type:Article
Date of Publication (online):2024/03/21
Date of first Publication:2023/10/23
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Open Access Fonds
anesthetics; bladder cancer; extracellular vesicles; miRNAs
Issue:20, Artikel 2503
First Page:2503-1
Last Page:2503-17
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum.
Institutes/Facilities:Marienhospital Herne, Klinik für Anästhesiologie, operative Intensivmedizin, Schmerz- und Palliativmedizin
Dewey Decimal Classification:Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit
open_access (DINI-Set):open_access
Licence (English):License LogoCreative Commons - CC BY 4.0 - Attribution 4.0 International