Dual-temperature microbiological control of cellular products

  • An experimental study by the Paul-Ehrlich Institute (PEI) demonstrated that temperatures between 35 and 37 °C are too high for the growth of some bacterial strains (e.g., \(\textit {Pseudomonas fluorescens}\)), leading to false negative results. Thus, the question of whether it is necessary to adapt incubation temperatures for the microbiological control of blood products, especially platelet concentrates (PCs), to enhance safety and regulatory compliance has arisen. In order to further elucidate this issue, the growth capability of different bacterial strains of interest in PCs and the detection efficacy of cultivation of these at different incubation temperatures must be taken into account. Therefore, we inoculated PCs with 46 different strains (3–6 PCs from different donors per strain) from different origins (PC isolates, reference strains) and stored PCs at 20–22 °C under constant agitation. On day three of storage, the inoculated PCs were sampled; aerobic and anaerobic culture bottles (BacT/Alert AST/NST) were each inoculated with 5 mL of sample, and culture bottles were incubated at 25 and 35 °C using the automated BacT/Alert Dual-temperature system. Bacterial proliferation was enumerated using a colony-forming assay. All strains of \(\it Enterobacteriacae\) (\(\it n\) = 5), \(\it Staphy-lococcus\) spp. (\(\it n\) = 11), \(\it Streptococcus\) spp. (\(\it n\) = 5), and \(\it Bacillus\) spp. (\(\it n\) = 4) and most \(\textit {Pseudomonas aeruginosa}\) strains (4 of 5) tested showed the capability to grow in most inoculated PCs, revealing a faster time to detection (TTD) at an incubation temperature of 35 °C. The tested \(\textit {Pseudomonas putida}\) (\(\it n\) = 3) strains showed a noticeably reduced capability to grow in PCs. Nonetheless, those with a notable growth capability revealed a faster TTD at an incubation temperature of 35 °C. Only one of the four \(\textit {Pseudomonas fluorescens}\) strains tested (strain ATCC 13525) was able to grow in PCs, showing a faster TTD at an incubation temperature of 25 °C but also detection at 35 °C. The commonly detected bacteria involved in the bacterial contamination of PCs showed a superior TTD at 35 °C incubation. Only one \(\textit {P. fluorescens}\) strain showed superior growth at 25 °C; however, the microbiological control at 35 °C did not fail to identify this contamination. In conclusion, the use of PC screening using a dual-temperature setting for microbiological control is presently not justified according to the observed kinetics.

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Author:Tanja VollmerGND, Cornelius KnabbeGND, Jens DreierGND
Parent Title (English):Microorganisms
Subtitle (English):a potential impact for bacterial screening of platelet concentrates?
Place of publication:Basel
Document Type:Article
Date of Publication (online):2024/03/13
Date of first Publication:2023/09/20
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Open Access Fonds
bacterial safety; dual-temperature; platelet concentrates
Issue:9, Article 2350
First Page:2350-1
Last Page:2350-12
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum.
Institutes/Facilities:Herz- und Diabeteszentrum NRW
Dewey Decimal Classification:Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit
open_access (DINI-Set):open_access
Licence (English):License LogoCreative Commons - CC BY 4.0 - Attribution 4.0 International