Applicability of chromatographic co-elution for antibiotic target identification

  • Identification of the molecular target is a crucial step in evaluating novel antibiotics. To support target identification, a label-free method based on chromatographic co-elution has previously been developed. Target identification by chromatographic coelution (TICC) exploits the alteration of the elution profile of target-bound drug versus free drug in ion exchange (IEX) chromatography to identify potential target proteins from elution fractions. The applicability of TICC for antibiotic research is investigated by evaluating which proteins, that is, putative targets, can be monitored in \(\textit {Bacillus subtilis}\). Coelution of components of known protein complexes provides a read-out for how well the native state of proteins is conserved during chromatography. Rifampicin, which targets RNA polymerase, is used in a proof-of-concept study.

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Author:Sina SchäkermannORCiDGND, Dominik WüllnerORCiDGND, Abdulkadir YayciORCiDGND, Andrew EmiliORCiDGND, Julia BandowORCiDGND
Parent Title (English):Proteomics
Place of publication:Weinheim
Document Type:Article
Date of Publication (online):2024/02/23
Date of first Publication:2020/09/19
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Bacillus subtilis; mode of action; protein complexes; protein-protein interaction; rifampicin
Issue:1, Artikel 2000038
First Page:2000038-1
Last Page:2000038-5
Dieser Beitrag ist auf Grund des DEAL-Wiley-Vertrages frei zugänglich.
Institutes/Facilities:Fakultät für Biologie und Biotechnologie, Arbeitsgruppe Angewandte Mikrobiologie
Dewey Decimal Classification:Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie
open_access (DINI-Set):open_access
faculties:Fakultät für Biologie und Biotechnologie
Licence (English):License LogoCreative Commons - CC BY-NC 4.0 - Attribution-NonCommercial 4.0 International