From \(\textit {in vitro}\) towards \(\textit {in situ}\)

  • ATP-binding cassette (ABC) exporters have been studied now for more than four decades, and recent structural investigation has produced a large number of protein database entries. Yet, important questions about how ABC exporters function at the molecular level remain debated, such as which are the molecular recognition hotspots and the allosteric couplings dynamically regulating the communication between the catalytic cycle and the export of substrates. This conundrum mainly arises from technical limitations confining all research to \(\textit {in vitro}\) analysis of ABC transporters in detergent solutions or embedded in membrane-mimicking environments. Therefore, a largely unanswered question is how ABC exporters operate \(\textit {in situ}\), namely in the native membrane context of a metabolically active cell. This review focuses on novel mechanistic insights into type I ABC exporters gained through a unique combination of structure determination, biochemical characterization, generation of conformation-specific nanobodies/sybodies and double electron–electron resonance.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Author:Enrica BordignonORCiDGND, Markus A. SeegerORCiDGND, Laura GalazzoORCiDGND, Gianmarco MeierORCiDGND
Parent Title (English):FEBS Letters
Subtitle (English):structure-based investigation of ABC exporters by electron paramagnetic resonance spectroscopy
Place of publication:Weinheim
Document Type:Article
Date of Publication (online):2024/02/23
Date of first Publication:2020/11/20
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:ABC transporters; DEER; EPR; MTSL; detergent; inside–out vesicles; liposomes; nanobody; spin label; sybody
First Page:3839
Last Page:3856
Dieser Beitrag ist auf Grund des DEAL-Wiley-Vertrages frei zugänglich.
Dewey Decimal Classification:Naturwissenschaften und Mathematik / Chemie, Kristallographie, Mineralogie
open_access (DINI-Set):open_access
faculties:Fakultät für Chemie und Biochemie
Licence (English):License LogoCreative Commons - CC BY-NC-ND 4.0 - Attribution-NonCommercial-NoDerivatives 4.0 International