Optogenetic stimulation shapes dendritic trees of infragranular cortical pyramidal cells

  • Spontaneous or experimentally evoked activity can lead to changes in length and/or branching of neocortical pyramidal cell dendrites. For instance, an early postnatal overexpression of certain AMPA or kainate glutamate receptor subunits leads to larger amplitudes of depolarizing events driven by spontaneous activity, and this increases apical dendritic complexity. Whether stimulation frequency has a role is less clear. In this study, we report that the expression of channelrhodopsin2-eYFP was followed by a 5-day optogenetic stimulation from DIV 5–10 or 11–15 in organotypic cultures of rat visual cortex-evoked dendritic remodeling. Stimulation at 0.05 Hz, at a frequency range of spontaneous calcium oscillations known to occur in the early postnatal neocortex \(\textit {in vivo}\) until eye opening, had no effect. Stimulation with 0.5 Hz, a frequency at which the cortex in vivo adopts after eye opening, unexpectedly caused shorter and somewhat less branched apical dendrites of infragranular pyramidal neurons. The outcome resembles the remodeling of corticothalamic and callosal projection neurons of layers VI and V, which in the adult have apical dendrites no longer terminating in layer I. Exposure to 2.5 Hz, a frequency not occurring naturally during the time windows, evoked dendritic damage. The results suggested that optogenetic stimulation at a biologically meaningful frequency for the selected developmental stage can influence dendrite growth, but contrary to expectation, the optogenetic stimulation decreased dendritic growth.

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Author:Steffen GondaORCiDGND, Ina GasterstädtORCiDGND, André HaaseORCiDGND, Katrin CzubayORCiDGND, Andrea RäkGND, Christian RiedelORCiDGND, Petra WahleORCiDGND
Parent Title (English):Frontiers in cellular neuroscience
Publisher:Frontiers Media
Place of publication:Lausanne
Document Type:Article
Date of Publication (online):2024/02/19
Date of first Publication:2023/08/01
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:Open Access Fonds
c-Fos; dendritic injury; dendritic retraction; postnatal development; rat visualcortex
Issue:Artikel 1212483
First Page:1212483-1
Last Page:1212483-13
Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum.
Institutes/Facilities:Lehrstuhl für Allgemeine Zoologie und Neurobiologie, Arbeitsgruppe für Entwicklungsneurobiologie
Dewey Decimal Classification:Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie
open_access (DINI-Set):open_access
faculties:Fakultät für Biologie und Biotechnologie
Licence (English):License LogoCreative Commons - CC BY 4.0 - Attribution 4.0 International