Characterization of a fold in TANGO1 evolved from SH3 domains for the export of bulky cargos
- Bulky cargos like procollagens, apolipoproteins, and mucins exceed the size of conventional COPII vesicles. During evolution a process emerged in metazoans, predominantly governed by the TANGO1 protein family, that organizes cargo at the exit sites of the endoplasmic reticulum and facilitates export by the formation of tunnel-like connections between the ER and Golgi. Hitherto, cargo-recognition appeared to be mediated by an SH3-like domain. Based on structural and dynamic data as well as interaction studies from NMR spectroscopy and microscale thermophoresis presented here, we show that the luminal cargo-recognition domain of TANGO1 adopts a new functional fold for which we suggest the term MOTH (MIA, Otoraplin, TALI/TANGO1 homology) domain. These MOTH domains, as well as an evolutionary intermediate found in invertebrates, constitute a distinct domain family that emerged from SH3 domains and acquired the ability to bind collagen.
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| Author: | Oliver ArnoldsORCiD, Raphael StollORCiDGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-107527 |
| DOI: | https://doi.org/10.1038/s41467-023-37705-4 |
| Parent Title (English): | Nature communications |
| Publisher: | Nature Publishing Group UK |
| Place of publication: | London |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2024/02/08 |
| Date of first Publication: | 2023/04/20 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | Open Access Fonds |
| Volume: | 14 |
| Issue: | Article 2273 |
| First Page: | 2273-1 |
| Last Page: | 2273-12 |
| Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
| Institutes/Facilities: | Lehrstuhl Biochemie II, Arbeitsgruppe Biomolekulare NMR-Spektroskopie |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Chemie, Kristallographie, Mineralogie |
| open_access (DINI-Set): | open_access |
| faculties: | Fakultät für Chemie und Biochemie |
| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
