Microscale atmospheric pressure plasma jet as a source for plasma-driven biocatalysis

  • The use of a microscale atmospheric pressure plasma jet (\(\mu\)APPJ) was investigated for its potential to supply hydrogen peroxide in biocatalysis. Compared to a previously employed dielectric barrier discharge (DBD), the \(\mu\)APPJ offered significantly higher H\(_2\)O\(_2\) production rates and better handling of larger reaction volumes. The performance of the \(\mu\)APPJ was evaluated with recombinant unspecific peroxygenase from \(\textit {Agrocybe aegerita}\) (r\(\it Aae\)UPO). Using plasma-treated buffer, no side reactions with other plasma-generated species were detected. For long-term treatment, r\(\it Aae\)UPO was immobilized, transferred to a rotating bed reactor, and reactions performed using the \(\mu\)APPJ. The enzyme had a turnover of 36,415 mol mol\(^{−}\)1 and retained almost full activity even after prolonged plasma treatment. Overall, the \(\mu\)APPJ presents a promising plasma source for plasma-driven biocatalysis.

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Metadaten
Author:Abdulkadir YayciORCiDGND, Tim DirksGND, Friederike KogelheideORCiDGND, Miguel AlcaldeORCiDGND, Frank HollmannORCiDGND, Peter AwakowiczORCiDGND, Julia E. BandowORCiDGND
URN:urn:nbn:de:hbz:294-99924
DOI:https://doi.org/10.1002/cctc.202001225
Parent Title (English):ChemCatChem
Publisher:Wiley-VCH
Place of publication:Hoboken, New Jersey
Document Type:Article
Language:English
Date of Publication (online):2023/06/23
Date of first Publication:2020/08/27
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:atmospheric pressure plasma; biocatalysis; hydrogen peroxide; peroxygenase; plasma chemistry
Volume:12
Issue:23
First Page:5893
Last Page:5897
Note:
Dieser Beitrag ist auf Grund des DEAL-Wiley-Vertrages frei zugänglich.
Institutes/Facilities:Fakultät für Biologie und Biotechnologie, Arbeitsgruppe Angewandte Mikrobiologie
Dewey Decimal Classification:Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie
open_access (DINI-Set):open_access
faculties:Fakultät für Biologie und Biotechnologie
Licence (English):License LogoCreative Commons - CC BY-NC 4.0 - Attribution-NonCommercial 4.0 International

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