Electroenzymatic nitrogen fixation using a MoFe protein system immobilized in an organic redox polymer
- We report an organic redox-polymer-based electroenzymatic nitrogen fixation system using a metal-free redox polymer, namely neutral-red-modified poly(glycidyl methacrylate-co-methylmethacrylate-co-poly(ethyleneglycol)methacrylate) with a low redox potential of −0.58 V vs. SCE. The stable and efficient electric wiring of nitrogenase within the redox polymer matrix enables mediated bioelectrocatalysis of N\(_3^{−}\), NO\(_2^{−}\) and N\(_2\) to NH\(_3\) catalyzed by the MoFe protein via the polymer-bound redox moieties distributed in the polymer matrix in the absence of the Fe protein. Bulk bioelectrosynthetic experiments produced 209\(\pm\)30 nmol NH\(_3\) nmol MoFe\(^{−1}\) h\(^{−1}\) from N\(_2\) reduction. \(^{15}N_2\) labeling experiments and NMR analysis were performed to confirm biosynthetic N\(_2\) reduction to NH\(_3\).
| Author: | Yoo Seok LeeGND, Adrian RuffORCiDGND, Rong CaiGND, Koun LimGND, Wolfgang SchuhmannORCiDGND, Shelley D. MinteerORCiDGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-98948 |
| DOI: | https://doi.org/10.1002/anie.202007198 |
| Parent Title (German): | Angewandte Chemie International Edition |
| Publisher: | Wiley-VCH |
| Place of publication: | Hoboken, New Jersey |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2023/05/19 |
| Date of first Publication: | 2020/06/04 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | ammonia; bioelectrosynthesis; neutral red; nitrogenase; redox polymers |
| Volume: | 59 |
| Issue: | 38 |
| First Page: | 16511 |
| Last Page: | 16516 |
| Note: | Dieser Beitrag ist auf Grund des DEAL-Wiley-Vertrages frei zugänglich. |
| Institutes/Facilities: | Lehrstuhl für Analytische Chemie - Elektroanalytik & Sensorik |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Chemie, Kristallographie, Mineralogie |
| open_access (DINI-Set): | open_access |
| faculties: | Fakultät für Chemie und Biochemie |
| Licence (English): |  Creative Commons - CC BY 4.0 - Attribution 4.0 International |
