Dielectric barrier discharge plasma treatment affects stability, metal ion coordination, and enzyme activity of bacterial superoxide dismutases

  • A molecular-level understanding of the effects of atmospheric-pressure plasma on biological samples requires knowledge of the effects on proteins. Superoxide dismutases, which detoxify superoxide under oxidative stress conditions, play a key role in bacterial plasma resistance. Investigation of the impact of dielectric barrier discharge (DBD) treatment on purified superoxide dismutases SodA and SodB of \(\textit {Escherichia coli}\) showed that DBD treatment caused a rapid protein degradation, with only 8% of protein remaining after 10 min. The affinity of SodA for the metal cofactor Mn\(^{2+}\) was reduced. Mass spectrometry, in conjunction with coupled-cluster calculations, revealed that modifications of amino acid residues in the active site can explain the decreased metal affinity and a distortion of the coordination geometry responsible for the activity loss.

Download full text files

Export metadata

Additional Services

Share in Twitter Search Google Scholar
Metadaten
Author:Marco KrewingORCiDGND, Christoph K. JungGND, Elena DobbelsteinGND, Britta SchubertGND, Timo JacobORCiDGND, Julia E. BandowORCiDGND
URN:urn:nbn:de:hbz:294-98898
DOI:https://doi.org/10.1002/ppap.202000019
Parent Title (English):Plasma processes and polymers
Publisher:Wiley-VCH
Place of publication:Hoboken, New Jersey
Document Type:Article
Language:English
Date of Publication (online):2023/05/16
Date of first Publication:2020/05/27
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Tag:iron; manganese; mass spectrometry; peroxynitrite; plasma medicine
Volume:17
Issue:10, Artikel 2000019
First Page:2000019-1
Last Page:2000019-14
Note:
Dieser Beitrag ist auf Grund des DEAL-Wiley-Vertrages frei zugänglich.
Institutes/Facilities:Fakultät für Biologie und Biotechnologie, Arbeitsgruppe Angewandte Mikrobiologie
Dewey Decimal Classification:Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie
open_access (DINI-Set):open_access
faculties:Fakultät für Biologie und Biotechnologie
Licence (English):License LogoCreative Commons - CC BY-NC 4.0 - Attribution-NonCommercial 4.0 International

OPUS4 Logo