Serum granulocyte-macrophage colony-stimulating factor (GM-CSF) is increased in patients with active radiographic axial spondyloarthritis and persists despite anti-TNF treatment
- \(\bf Background:\) Accumulating evidence supports the role of monocytes and neutrophils in radiographic axSpA (r-axSpA). Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a growth factor for both leukocyte lineages and a pro-inflammatory cytokine activating myeloid cells and promoting osteoclastogenesis. It acts through the JAK-STAT pathway. We measured serum GM-CSF and markers of bone metabolism in patients with r-axSpA before and after anti-TNF treatment. \(\bf Methods:\) Patients with active r-axSpA despite treatment with NSAIDs, all eligible for treatment with a biologic agent, were recruited. Healthy donors were sampled as controls. Serum was collected before (baseline) and after 4–6 months (follow-up) of anti-TNF treatment and the following molecules were measured with ELISA: GM-CSF, sclerostin (SOST), and dickkopf-1 (Dkk-1). \(\bf Results:\) Twelve r-axSpA patients (7 males, 5 females, median age 37 years) with a median disease duration of 1 year and 16 age- and sex-matched controls were included. At baseline, patients had mean BASDAI 6.3\(\pm\)2 and ASDAS 3.2\(\pm\)0.7, which decreased to 4.1\(\pm\)1.7 and 2.2\(\pm\)0.6 at follow-up, respectively. At baseline, r-axSpA patients had significantly higher mean serum levels of GM-CSF (150 vs 62pg/ml, \(\it p\)=0.049), significantly lower Dkk-1 (1228 vs 3052pg/ml, \(\it p\)=0.001), but similar levels of SOST (369 vs 544pg/ml, \(\it p\)=0.144) compared to controls. Anti-TNF treatment did not affect GM-CSF, Dkk-1, or SOST levels. Spearman correlation analysis showed that GM-CSF correlated positively with ASDAS at baseline (\(\it r\)=0.61, \(\it p\)=0.039), while no correlations were identified between bone markers (Dkk-1, SOST) on one hand and GM-CSF or disease activity indices on the other. \(\bf Conclusions:\) GM-CSF is increased in patients with active AS and strongly correlates with disease activity. TNF inhibition does not affect GM-SCF levels, despite improving disease activity. GM-CSF may represent an important pathway responsible for residual inflammation during TNF blockade, but also a potential target of JAK inhibitors, explaining their efficacy in r-axSpA.
Author: | Charalampos PapagorasORCiDGND, Styliani TsiamiORCiDGND, Akrivi ChrysanthopoulouORCiDGND, Ioannis MitroulisORCiDGND, Xenofon BaraliakosORCiDGND |
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URN: | urn:nbn:de:hbz:294-104049 |
DOI: | https://doi.org/10.1186/s13075-022-02888-6 |
Parent Title (English): | Arthritis research & therapy |
Publisher: | BioMed Central |
Place of publication: | London, Vereinigtes Königreich |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2023/11/16 |
Date of first Publication: | 2022/08/16 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | Open Access Fonds Granulocyte-monocyte colony-stimulating factor; Neutrophils; Radiographic axial spondyloarthritis |
Volume: | 24 |
Issue: | Article 195 |
First Page: | 195-1 |
Last Page: | 195-5 |
Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
Institutes/Facilities: | Rheumazentrum Ruhrgebiet |
Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
open_access (DINI-Set): | open_access |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |