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Pádraic J. Flood, Minou Nowrousian, Bruno Hüttel, Christian Wöhle, Kerstin Becker, Tassilo Erik Wollenweber, Dominik Begerow, Christopher Grefen

• \(\bf Objectives:\) \(\textit {Lathyrus tuberosus}\) is a nitrogen-fixing member of the Fabaceae which forms protein-rich tubers. To aid future domestication programs for this legume plant and facilitate evolutionary studies of tuber formation, we have generated a draft genome assembly based on Pacific Biosciences sequence reads. \(\textbf {Data description:}\) Genomic DNA from \(\textit {L. tuberosus}\) was sequenced with PacBio's HiFi sequencing chemistry generating 12.8 million sequence reads with an average read length of 14 kb (approximately 180 Gb of sequence data). The reads were assembled to give a draft genome of 6.8 Gb in 1353 contigs with an N50 contig length of 11.1 Mb. The GC content of the genome assembly was 38.3%. BUSCO analysis of the genome assembly indicated a genome completeness of at least 96%. The genome sequence will be a valuable resource, for example, in assessing genomic consequences of domestication efforts and developing marker sets for breeding programs. The \(\textit {L. tuberosus}\) genome will also aid in the analysis of the evolutionary history of plants within the nitrogen-fixing Fabaceae family and in understanding the molecular basis of tuber evolution.