The predatory soil bacterium \(\textit {Myxococcus xanthus}\) combines a Tad- and an atypical type 3-like protein secretion system to kill bacterial cells
- Predatory Myxobacteria employ a multilayered predation strategy to kill and lyse soil microorganisms. Aiming to dissect the mechanism of contact-dependent killing of bacteria, we analyze four protein secretion systems in \(\textit {Myxococcus xanthus}\) and investigate the predation of mutant strains on different timescales. We find that a Tad-like and a type 3-like secretion system (Tad and T3SS∗) fulfill distinct functions during contact-dependent prey killing: the Tad-like system is necessary to induce prey cell death, while the needle-less T3SS∗ initiates prey lysis. Fluorescence microscopy reveals that components of both systems interdependently localize to the predator-prey contact site prior to killing. Swarm expansion assays show that both Tad and T3SS∗ are required to handle live prey and that nutrient extraction from prey bacteria is sufficient to power \(\textit {M. xanthus}\) motility. In conclusion, our observations indicate the functional interplay of two types of secretion systems for killing and lysis of bacterial cells.
| Author: | Susanne ThieryGND, Pia TurowskiGND, James E. BerlemanORCiDGND, Christine KaimerORCiDGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-102783 |
| DOI: | https://doi.org/10.1016/j.celrep.2022.111340 |
| Parent Title (English): | Cell reports |
| Publisher: | Cell Press |
| Place of publication: | Maryland Heights, Missouri, USA |
| Document Type: | Article |
| Language: | English |
| Date of Publication (online): | 2023/10/24 |
| Date of first Publication: | 2022/09/13 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Tag: | Open Access Fonds |
| Volume: | 40 |
| Issue: | 11, Article 111340 |
| First Page: | 111340-1 |
| Last Page: | 111340-12, e4, 12 |
| Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
| Institutes/Facilities: | Lehrstuhl für Biologie der Mikroorganismen |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| open_access (DINI-Set): | open_access |
| faculties: | Fakultät für Biologie und Biotechnologie |
| Licence (English): |  Creative Commons - CC BY-NC-ND 4.0 - Attribution-NonCommercial-NoDerivatives 4.0 International |
