The emergence of embryonic myosin heavy chain during branchiomeric muscle development
- A prerequisite for discovering the properties and therapeutic potential of branchiomeric muscles is an understanding of their fate determination, pattering and differentiation. Although the expression of differentiation markers such as myosin heavy chain (MyHC) during trunk myogenesis has been more intensively studied, little is known about its expression in the developing branchiomeric muscle anlagen. To shed light on this, we traced the onset of MyHC expression in the facial and neck muscle anlagen by using the whole-mount in situ hybridization between embryonic days E9.5 and E15.5 in the mouse. Unlike trunk muscle, the facial and neck muscle anlagen express MyHC at late stages. Within the branchiomeric muscles, our results showed variation in the emergence of MyHC expression. MyHC was first detected in the first arch-derived muscle anlagen, while its expression in the second arch-derived muscle and non-somitic neck muscle began at a later time point. Additionally, we show that non-ectomesenchymal neural crest invasion of the second branchial arch is delayed compared with that of the first brachial arch in chicken embryos. Thus, our findings reflect the timing underlying branchiomeric muscle differentiation.
Author: | Imadeldin YahyaORCiDGND, Marion BöingGND, Dorit HockmanORCiDGND, Beate Brand-SaberiORCiDGND, Gabriela Morosan-PuopoloORCiDGND |
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URN: | urn:nbn:de:hbz:294-102141 |
DOI: | https://doi.org/10.3390/life12060785 |
Parent Title (English): | Life |
Publisher: | MDPI |
Place of publication: | Basel |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2023/09/29 |
Date of first Publication: | 2022/05/25 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | Open Access Fonds MyHC; branchiomeric muscles; chicken embryos; mouse embryos; neck muscles; non-ectomesenchymal crest cells |
Volume: | 16 |
Issue: | 6, Article 785 |
First Page: | 785-1 |
Last Page: | 785-13 |
Note: | Article Processing Charge funded by the Deutsche Forschungsgemeinschaft (DFG) and the Open Access Publication Fund of Ruhr-Universität Bochum. |
Institutes/Facilities: | Institut für Anatomie, Abteilung für Anatomie und Molekulare Embryologie |
Institut für Anatomie | |
Dewey Decimal Classification: | Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit |
open_access (DINI-Set): | open_access |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |