Sarah Plum, Britta Eggers, Stefan Helling, Markus Stepath, Carsten Theiß, Renata E.P. Leite, Mariana Molina, Lea T. Grinberg, Peter Riederer, Manfred Gerlach, Caroline May, Katrin Marcus-Alic
- The pathological hallmark of Parkinson's disease (PD) is the loss of neuromelanin-containing dopaminergic neurons within the substantia nigra pars compacta (SNpc). Additionally, numerous studies indicate an altered synaptic function during disease progression. To gain new insights into the molecular processes underlying the alteration of synaptic function in PD, a proteomic study was performed. Therefore, synaptosomes were isolated by density gradient centrifugation from SNpc tissue of individuals at advanced PD stages (N = 5) as well as control subjects free of pathology (N = 5) followed by mass spectrometry-based analysis. In total, 362 proteins were identified and assigned to the synaptosomal core proteome. This core proteome comprised all proteins expressed within the synapses without regard to data analysis software, gender, age, or disease. The differential analysis between control subjects and PD cases revealed that CD9 antigen was overrepresented and fourteen proteins, among them Thymidine kinase 2 (TK2), mitochondrial, 39S ribosomal protein L37, neurolysin, and Methionine-tRNA ligase (MARS2) were underrepresented in PD suggesting an alteration in mitochondrial translation within synaptosomes.
MetadatenAuthor: | Sarah PlumGND, Britta EggersORCiDGND, Stefan HellingGND, Markus StepathGND, Carsten TheißORCiDGND, Renata E.P. LeiteGND, Mariana MolinaGND, Lea T. GrinbergGND, Peter RiedererGND, Manfred GerlachGND, Caroline MayORCiDGND, Katrin Marcus-AlicORCiDGND |
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URN: | urn:nbn:de:hbz:294-79969 |
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DOI: | https://doi.org/10.3390/cells9122580 |
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Parent Title (English): | Cells |
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Publisher: | MDPI |
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Place of publication: | Basel |
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Document Type: | Article |
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Language: | English |
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Date of Publication (online): | 2021/04/09 |
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Date of first Publication: | 2020/12/02 |
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Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
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Tag: | Parkinson's disease; mitochondrial pathology; mitochondrial translation; proteomics; substantia nigra pars compacta; synaptosomes |
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Volume: | 9 |
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Issue: | 12, Article 2580 |
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First Page: | 2580-1 |
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Last Page: | 2580-19 |
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Note: | Supplementary File 1 (ZIP, 4119 KiB): https://www.mdpi.com/2073-4409/9/12/2580/s1 |
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Institutes/Facilities: | Medizinisches Proteom-Center, Forschungsbereich Funktionelle Proteomik |
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| Research Department of Neuroscience |
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| Protein Research Department |
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open_access (DINI-Set): | open_access |
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faculties: | Medizinische Fakultät |
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Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |
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