Do cultured human skin explants elaborate coeliac antigen, possibly even Tissue-Transglutaminase?

  • To satisfy the demand for the most specific form of human tTG to use it as antigenic substrate in ELISA-tests, fibroblasts derived from skin biopsies of CD patients and healthy control patients were cultivated. Possible antigens in the medium that they produced and in sonicates of the fibroblasts themselves were seperated by SDS-Polyacrylamide gel electrophoresis with subsequent electrophoretic transfer to nitrocellulose membranes. In the following western blot they were exposed to serum of other untreated CD, treated CD and healthy control patients and the molecular weight of antigens was identified and compared to tTG. In conclusion cultured human skin explants do not secrete an antigen against CD antibodies into their environment. However the presence of such an antigen can be observed in fibroblast sonicates after the incubation with some CD sera. Whether this antigen of the molecular size of 20 - 25 kDa could be related to a fragment of tTG has to be evaluated in further studies.

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Author:Claus ReinsbergerGND
URN:urn:nbn:de:hbz:294-6654
Referee:Burkard MayGND, A.K. Stephan El-GammalGND
Document Type:Doctoral Thesis
Language:English
Date of Publication (online):2003/03/18
Date of first Publication:2003/03/18
Publishing Institution:Ruhr-Universität Bochum, Universitätsbibliothek
Granting Institution:Ruhr-Universität Bochum, Medizinische Fakultät
Date of final exam:2002/12/17
Creating Corporation:Medizinische Fakultät
GND-Keyword:Zöliakie; Fibroblast; Serologie; Autoantigen; Diagnostik
Institutes/Facilities:Berufsgenossenschaftliches Universitätsklinikum Bergmannsheil, Medizinische Klinik I, Abteilung für Gastroenterologie und Hepatologie
Dewey Decimal Classification:Technik, Medizin, angewandte Wissenschaften / Medizin, Gesundheit
faculties:Medizinische Fakultät
Licence (German):License LogoKeine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht

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