\(\textit {From lab to lake}\)
- Quantitative PCR methods are commonly used to monitor enteric viruses in the aquatic environment because of their high sensitivity, short reaction times and relatively low operational cost. However, conclusions for public health drawn from results of such molecular techniques are limited due to their inability to determine viral infectivity. Ethidium monoazide (EMA) and propidium monoazide (PMA) are capable to penetrate the damaged or compromised capsid of the inactivated viruses and bind to the viral nucleic acids. We assessed whether dye treatment is a suitable approach to improve the ability of qPCR to distinguish between infectious and non-infectious human adenovirus, enterovirus and rotavirus A in surface water of an urban river and sewage before and after UV disinfection. Like the gold standard of cell culture assays, pretreatment EMA-/PMA-qPCR succeeded in removing false positive results which would lead to an overestimation of the viral load if only qPCR of the environmental samples was considered. A dye pretreatment could therefore provide a rapid and relatively inexpensive tool to improve the efficacy of molecular quantification methods in regards to viral infectivity.
Author: | Mats LeifelsGND, Ibrahim Ahmed HamzaGND, Marion KriegerGND, Michael WilhelmORCiDGND, Martin MackowiakGND, Lars JurzikORCiDGND |
---|---|
URN: | urn:nbn:de:hbz:294-58530 |
DOI: | https://doi.org/10.1371/journal.pone.0167105 |
Parent Title (English): | PLoS one |
Subtitle (English): | evaluation of current molecular methods for the detection of infectious enteric viruses in complex water matrices in an urban area |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2018/07/05 |
Date of first Publication: | 2016/11/23 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | Open Access Fonds |
Volume: | 11 |
Issue: | 11 |
First Page: | e0167105-1 |
Last Page: | e0167105-16 |
Note: | Article Processing Charge funded by the Open Access Publication Fund of Ruhr-Universität Bochum. |
Note: | PLoS ONE, Bd. 11.2016, H. 11, Artikelnummer e0167105 |
Institutes/Facilities: | Institut für Hygiene und Mikrobiologie, Abteilung für Hygiene, Sozial- und Umweltmedizin |
Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
open_access (DINI-Set): | open_access |
faculties: | Medizinische Fakultät |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |