Two distinct cardiolipin synthases operate in \(\textit {Agrobacterium tumefaciens}\)
- Cardiolipin (CL) is a universal component of energy generating membranes. In most bacteria, it is synthesized via the condensation of two molecules phosphatidylglycerol (PG) by phospholipase D-type cardiolipin synthases (PLD-type Cls). In the plant pathogen and natural genetic engineer \(\textit {Agrobacterium tumefaciens}\) CL comprises up to 15% of all phospholipids in late stationary growth phase. \(\textit {A. tumefaciens}\) harbors two genes, \(\it atu1630\) (\(\it cls1\)) and \(\it atu2486\) (\(\it cls2\)), coding for PLD-type Cls. Heterologous expression of either \(\it cls1\) or \(\it cls2\) in \(\textit {Escherichia coli}\) resulted in accumulation of CL supporting involvement of their products in CL synthesis. Expression of \(\it cls1\) and \(\it cls2\) in \(\textit {A. tumefaciens}\) is constitutive and irrespective of the growth phase. Membrane lipid profiling of \(\textit {A. tumefaciens}\) mutants suggested that Cls2 is required for CL synthesis at early exponential growth whereas both Cls equally contribute to CL production at later growth stages. Contrary to many bacteria, which suffer from CL depletion, \(\textit {A. tumefaciens}\) tolerates large changes in CL content since the CL-deficient \(\it cls1/cls2\) double mutant showed no apparent defects in growth, stress tolerance, motility, biofilm formation, UV-stress and tumor formation on plants.
Author: | Simon CzolkossGND, Christiane FritzGND, Georg HölzlGND, Meriyem AktasORCiDGND |
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URN: | urn:nbn:de:hbz:294-58412 |
DOI: | https://doi.org/10.1371/journal.pone.0160373 |
Parent Title (English): | PLoS one |
Document Type: | Article |
Language: | English |
Date of Publication (online): | 2018/07/04 |
Date of first Publication: | 2016/07/29 |
Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
Tag: | Open Access Fonds |
Volume: | 11 |
Issue: | 7 |
First Page: | e0160373-1 |
Last Page: | e0160373-18 |
Note: | Article Processing Charge funded by the Open Access Publication Fund of Ruhr-Universität Bochum. |
Note: | PLoS ONE, Bd. 11.2016, H. 7, Artikelnummer e0160373 |
Institutes/Facilities: | Lehrstuhl für Biologie der Mikroorganismen |
Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
open_access (DINI-Set): | open_access |
faculties: | Fakultät für Biologie und Biotechnologie |
Licence (English): | Creative Commons - CC BY 4.0 - Attribution 4.0 International |