RUB » Bibliotheksportal

Denis A. Akkad, Wanda Maria Gerding, Robin B. Gasser, Jörg T. Epplen

• \(\textbf{Background:}\) The domestic dog represents an important model for studying the genetics of behavior. In spite of technological advances in genomics and phenomics, the genetic basis of most specific canine behaviors is largely unknown. Some breeds of hunting dogs exhibit a behavioral trait called "pointing" (a prolonged halt of movement to indicate the position of a game animal). Here, the genomes of pointing dogs (Large Munsterlander and Weimaraner) were compared with those of behaviorally distinct herding dogs (Berger des Pyrenées and Schapendoes). We assumed (i) that these four dog breeds initially represented inbred populations and (ii) that selective breeding for pointing behavior promotes an enrichment of the genetic trait in a homozygous state. \(\textbf{Results:}\) The homozygosity mapping of 52 dogs (13 of each of the four breeds) followed by subsequent intervalresequencing identified fixed genetic differences on chromosome 22 between pointers and herding dogs. In addition, we identified one non-synonomous variation in each of the coding genes \(\textit{SETDB2}\) and \(\textit{CYSLTR2}\) that might have a functional consequence. Genetic analysis of additional hunting and non-hunting dogs revealed consistent homozygosity for these two variations in six of seven pointing breeds. \(\textbf{Conclusions:}\) Based on the present findings, we propose that, together with other genetic, training and/or environmental factors, the nucleotide and associated amino acid variations identified in genes \(\textit{SETDB2}\) and \(\textit{CYSLTR2}\) contribute to pointing behavior.