Development and characterization of simian immunodeficiency virusbased vectors for gene transfer and immunization
- To further address safety concerns of live attenuated viruses as preventive vaccines against HIV infection, a SIV-based single cycle immunodeficiency virus vaccine (SCIV) was developed. This was achieved by mutating the primer binding site of SIV genome to recognize complementing artificial tRNAs but not cellular tRNAs, leading to the impairment of the reverse transcription process \(\textit {in vivo}\) in the absence of artificial tRNAs. Immunization of rhesus monkeys with SCIVs could stimulate both antibody and cell-mediated immune responses. At the doses used for immunization however, these vaccines could not provide long-term control of the replication of pathogenic SIVs used to challenge the immunized animals. In order to determine if the establishment of packaging cell line for the production of large amount of SCIVs was possible, a SIV-based minimal packaging cell line was generated which could be used to produce up to \(10^{5}\) IU/ml of VSV-G pseudotyped lentiviral vectors.
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| Author: | Seraphin KuateGND |
|---|---|
| URN: | urn:nbn:de:hbz:294-6282 |
| Referee: | Klaus Thomas ÜberlaGND, Bernd-Joachim BeneckeGND |
| Document Type: | Doctoral Thesis |
| Language: | English |
| Date of Publication (online): | 2003/03/18 |
| Date of first Publication: | 2003/03/18 |
| Publishing Institution: | Ruhr-Universität Bochum, Universitätsbibliothek |
| Granting Institution: | Ruhr-Universität Bochum, Fakultät für Chemie und Biochemie |
| Date of final exam: | 2002/10/22 |
| Creating Corporation: | Fakultät für Chemie und Biochemie |
| GND-Keyword: | Affenimundefizienzvirus; Gentransfer; Immunisierung; HIV; Lentiviren |
| Institutes/Facilities: | Medizinische Fakultät, Abteilung für Molekulare und Medizinische Virologie |
| Dewey Decimal Classification: | Naturwissenschaften und Mathematik / Biowissenschaften, Biologie, Biochemie |
| faculties: | Fakultät für Chemie und Biochemie |
| Licence (German): |  Keine Creative Commons Lizenz - es gelten der Veröffentlichungsvertrag und das deutsche Urheberrecht |
